TSKgel FcR-IIIA-NPR

SerCoLab is happy to announce the launch of a new affinity column - TSKgel FcR-IIIA-NPR - based on a recombinant FcγIIIa receptor ligand. The column is designed for fast assessment for biologic activity of mAbs. The FcγIIIa receptor plays a key role in antibody-dependent cell mediated cytotoxicity (ADCC) by interacting with the N-glycans of the Fc domain of immunoglobulins. Hence, affinity chromatography using immobilized Fc receptor ligands can deliver valuable information about ADCC activity of therapeutic antibodies. As the interaction/affinity is influenced by N-glycans the separation profile also reflect the antibodies glycoform pattern.
There is no comparable HPLC column on the market. Highlights of the product are:
• Easy and reproducible HPLC analysis based on FcγRIIIa affinity of mAbs
• Unique glycoprotein elution profile of IgG allows assessment of ADCC activity
• Applicable to purified samples and cell culture supernatant alike
• Fast cell line screening, upstream development, lot-to-lot comparison
A rapid thirty-minute separation allows the analysis of large numbers of mAb samples to gain valuable first information on the distribution of glycoforms and expected ADCC activity. This fast and efficient method can be applied to purified samples and supernatant alike and can therefore be used in many phases of development and production such as cell line screening in early R&D, biosimilar/originator comparison, upstream development and optimization, monitoring of glycoengineering, or lot-to-lot comparison in QC.
TSKgel FcR-IIIA-NPR (4.6 mm ID, 7.5 cm L, 5 µm) is available at a very attractive, reduced introductory price (valid until end of 2019) and is packed in PEEK hardware.
More detailed information can be obtained from frank.vanderstraeten@sercolab.be

 

 

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