SerCoLab Newsletter - March 2021


Dear Reader,

In this edition of the SerCoLab Newsletter you can find the latest news from our partners
Knauer & Tosoh Bioscience.
A very interesting application note regarding the quick analysis of plasmid topoisomers.
You can also register for Tosoh's upcoming BioSeparation Forum Web Event via the link below.
Learn more about the new analytical HPLC system AZURA 862.

As the official distributor for Knauer and Tosoh in Belgium and Luxembourg,
SerCoLab is at your disposal for all your queries.

Subscribe to our newsletters and enjoy the read!
Analytical ion exchange chromatography on a TSKgel DNA-NPR column offers a simple and rapid method for discriminating between covalently closed circular (CCC), open circular (OC), and linear (L) forms of plasmid DNA.
Recombinant plasmid DNA (pDNA) is increasingly used as a raw material in gene therapy and as an active ingredient for DNA vaccination.
Pharmaceutical grade plasmid DNA must meet specifications concerning the content of pDNA topoisomers (supercoiled, open circular, and linear).
Read how an HPLC method with high resolving power employs the TSKgel DNA-NPR anion exchange HPLC column to rapidly analyze DNA topoisomers.
Used product:
TSKgel DNA-NPR, 2.5 μm, 4.6 mm ID x 7.5 cm L
(P/N 0018249)
Join TOSOH's BioSeparation Forum Web Event on March 11, 2021
Oligonucleotides in the Spotlight

Gain insights into chromatography applied in manufacturing & characterization of therapeutic oligonucleotides.
Register for the 1st virtual BioSeparation Forum in 2021 and attend inspiring presentations and scientific updates presented by experts from biopharma and Tosoh Bioscience.

Topics will cover use, synthesis, purification, and analysis of nucleic acid therapeutics with a focus on oligonucleotides.
Register Here !
More on AZURA 862
AZURA 862's upgradeability to ULDC is a great advantage
The new analytical HPLC system AZURA 862 raises the bar for standard HPLC, allowing separations at up to 12,500 psi / 862 bar.

Where is the advantage, when most of my HPLC applications run at pressures below 300 bar?

Replacing an old HPLC system with a most up-to-date AZURA 862 HPLC system, will give you high-performing components like the P 6.1L eluent supply, the flexible AS 6.1L autosampler, sensitive detectors with high data acquisition rates, and modern network connectivity today.

You can continue to run your routine applications at slightly higher performance and benefit from modern system features. The 862 bar pressure-resistance increases the number of modern small-particle columns applicable on your system. So, with AZURA 862 you will be able to use e.g. core-shell column materials;
Should your future methods require higher 
resolution, you can then easily upgrade your AZURA 862 with ULDC technology. This upgrade transforms the system into an ultra-low dispersion chromatography system, which will minimize the extra-column volume and provide UHPLC benefits.
The separation power of modern sub-2µm or core-shell columns can be fully utilized with ULDC resulting in higher resolution for sharper peaks or faster separations.

Figure 1: Separation of alkyl benzoates demonstrating possible speed improvement.
Figure 2: Alkyl benzoates separation, variation of extra-column volume (effect on efficiency)
Figure 3: Efficiency comparison of alkyl benzoates separation. Early eluting peaks benefit most.

AZURA 862 HPLC system:
  • Most up-to-date HPLC components, performance-wise, control-wise
  • Can easily replace existing systems running the same methods
  • Upgradable to UHPLC level performance
The ULDC option makes the new AZURA 862 a future-proof HPLC:
  • Ultra-low dispersion chromatography (ULDC) for the next level of HPLC
  • Ultra-low extra column volume
  • Maximized separation power with UHPLC benefits
An HPLC->ULDC adaptable system is not only practical from a cost perspective, but also more sustainable, because no systems will sit around unused, just due to an unexpected need for HPLC or UHPLC methods.
Contact us at SerCoLab for more information.

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