TSKgel® AMIDE-80 2 μm HILIC COLUMNS

TSKgel Amide-80 columns packed with 2 μm silica based particles are the latest addition to the well-known TSKgel Amide-80 series. The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction (HILIC) mode of chroma- tography. The Amide-80 phase shows higher retention of polar compounds than other Amide columns on the market.

For years, TSKgel Amide-80 columns are used success- fully for HILIC separations of polar compounds such as saccharides, glycans, oligosaccharides or peptides, documented in more than 250 scientific publications. In parallel to the growth of the biotherapeutics market the use of Amide-phases for glycosylation analysis steadily increased.

Packed with spherical silica particles that are covalently bonded with non-ionic carbamoyl groups (Figure 1), TSKgel Amide-80 provides higher stability than conven- tional amino-phases and a unique selectivity. Figure 2 shows the characterization of the new 2 μm version of TSKgel Amide-80 compared to the renowned 3 μm Amide-80 based on the system proposed by Y. Kawachi et al. (J. Chromatogr. A, 1218 (2011) 5903 ff).

The new 2 μm TSKgel Amide-80 material improves peak capacity and sensitivity for both, (U)HPLC and LC-MS analysis. When using short columns this can be exploited to considerably shorten analysis time. The columns are especially suited for use in UHPLC systems, as their reduced system volume and optimized detector specifi- cations help to maintain the high resolution that can be achieved with 2 micron columns.

HIGHLIGHTS
Proven TSKgel Amide-80 HILIC selectivity
Easy transfer of existing methods
Applicable to both HPLC and UHPLC
Optimized for (U)HPLC glycosylation analysis
Applicable for normal phase analysis